Assay summary for ChIP-seq_Skarmeta_Lab_H3K27ac_0002AS
Skarmeta Lab, Centro Andaluz de Biologia del Desarrollo
Libraries were prepared as described in (Bogdanovic et al, 2013, Methods): The samples were sonicated using the Diagenode Bioruptor device with the following cycling conditions: 15 min high–30 sec on, 30 sec off; 15 min on ice; 15 min high–30 sec on, 30 sec off. The size of sonicated DNA was in the range of 100–500 bp. Immunoprecipitated DNA was purified with QIA-quick columns (Qiagen). The DNA ends were repaired, and the adaptors ligated. The size-selected (300 bp) library was then amplified in a PCR reaction and sequenced using the Genome Analyzer (Illumina). The anti-H3K27ac antibody was purchased from Abcam (ab4729).