Assay summary for ChIP-seq_Wardle_Lab_Eomesa_0001AS
Wardle Lab, King's College London
ChIP was performed on 5,000 high-sphere embryos ( as described in Morley RH, Lachani K, Keefe D, Gilchrist MJ, Flicek P, Smith JC, Wardle FC: A gene regulatory network directed by zebrafish No tail accounts for its roles in mesoderm formation. Proc Natl Acad Sci U S A. 2009, 106: 3829-3834. 10.1073/pnas.080838210600. anti-Eomesa (50 μl) antibody were used. Illumina paired-end libraries were prepared according to the manufacturer's instructions, size selected and sequenced on the Illumina GAIIx platform (Illumina, San Diego, CA, USA).