Assay summary for ChIP-seq_Zon_Lab_Input_0003AS

Assay Type:
Zon Lab, Boston Children's Hospital
ChIPSeq_Input; Whole cell extracts were sonicated to solubilize the chromatin. The chromatin extracts containing DNA fragments with an average size of 1000 bp were immunoprecipitated using different antibodies. Purified immunoprecipitated DNA were prepared for sequencing according to a modified version of the Solexa Genomic DNA protocol. Fragmented DNA was end repaired and subjected to 18 cycles of LM-PCR using oligos provided by Illumina. Amplified fragments between 150 and 300bp (representing shear fragments between 50 and 200nt in length and ~100bp of primer sequence) were isolated by agarose gel electrophoresis and purified.
Added by User:
Date Added:
June 2, 2016, 3:02 p.m.
Last Edited:
July 25, 2018, 5:46 a.m.