Bobe lab, Laboratoire de Physiologie et Genomique des Poissons, INRA, Rennes
Sequencing libraries were prepared using a TruSeq RNA sample preparation kit, according to manufacturer instructions (Illumina, San Diego, CA). Poly-A-containing mRNA was isolated from total RNA using poly-T oligo-attached magnetic beads, and chemically fragmented. First-strand cDNA was generated using SuperScript II reverse transcriptase and random primers. Following the second strand cDNA synthesis and adaptor ligation, cDNA fragments were amplified by PCR. Products were loaded onto an Illumina HiSeq2000 instrument and subjected to multiplexed paired-end (2 × 100 bp) sequencing.