Assay summary for RNA-seq_Schier_Lab_0011AS

Assay Type:
Schier Lab, Harvard University
Total RNA was isolated using the standard Trizol (Invitrogen) protocol. Two rounds of PolyA+-RNA purification were performed for each sample, using the PolyA(Purist)TM-MAG kit (Ambion). The quality of the RNA and lack of contaminating ribosomal RNA were confirmed using the Agilent 2100 Bioanalyzer. Strand-specific libraries for 76 bp paired-end sequencing were prepared according to a modified UTP-method (Parkhomchuk et al. 2009), as detailed in (Levin et al. 2010). Libraries were prepared using the Illumina sequencing library preparation protocol.
Library Preparation:
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Date Added:
July 2, 2018, 8:38 a.m.
Last Edited:
July 2, 2018, 8:38 a.m.