Assay summary for RNA-seq_Skarmeta_Lab_0001AS

Assay Type:
Skarmeta Lab, Centro Andaluz de Biologia del Desarrollo
Total RNA was purified from tissue using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer instructions. mRNA-Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). Strand-specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al., High-Throughput Illumina Strand-Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011, 940-949 (2011)].
Library Preparation:
Added by User:
Date Added:
July 26, 2018, 5:22 a.m.
Last Edited:
Oct. 19, 2018, 8:38 a.m.