Assay summary for ChIP-seq_Shkumatava_Lab_H3K27ac_0001AS
Assay Type:
ChIP-seq
Lab:
Shkumatava Lab, Institut Curie
Description:
ChIP-seq was performed as previously described (Guenther et al. 2008) using 5 ug Abcam H3K27ac antibody (ab4729, lot# GR259887-1) bound to Dynal Protein A linked beads (Invitrogen). Reverse cross-linked and phenol:chloroform purified chromatin was used for single-end library preparation following standard Illumina protocols.
DNA fragments with an average size of 175 bp or 200 bp were used for single-end library preparation following standard Illumina protocols.