RNA was purified by trizol-chlorophorm extraction (ThermoFisher ID: 15596026) of whole embryos followed by column cleanup. Briefly, 20-200 embryos (for 4dpf and 6hpf respectively) were lysed in 500ul of Trizol, and following chloroform extraction, the RNA-containing aqueous phase was mixed 1:1 with 100% ethanol and bound to an RNAeasy column (Qiagen ID: 74104) by centrifugation according to manufacturerÕs recommendations. Column bound RNA was then washed with RW1 and RPE buffers and then eluted with water. Purified RNA was then prepared as libraries for sequencing using the TruSeq Stranded Total RNA Library Prep Kit with Ribo- Zero (Illumina ID: RS-122-2201). High-throughput sequencing was by IlluminaÕs protocol for 50bp single-end runs on an Illumina HiSeq 2500.