Skarmeta Lab, Centro Andaluz de Biologia del Desarrollo
Description:
Total RNA was purified from tissue using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer instructions. mRNA-Seq libraries were generated from total RNA with polyA+ selection of mRNA using the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA). Strand-specific libraries were constructed using a dUTP methodology as described previously [Zhong S. et al., High-Throughput Illumina Strand-Specific RNA Sequencing Library Preparation. Cold Spring Harb. Protoc. 2011, 940-949 (2011)].