Series type: Survey

Publication: 10.1126/science.1242527

GEO/SRA ID: GSE39780

Description: Zebrafish homologues of the mammalian core pluripotency factors, Pou5f3 and SoxB1 are also crucially involved in Zygotic Genome Activation in zebrafish. To identify the components of Pou5f3 and SoxB1-dependent transcriptional networks, Wolfgang Driever lab determined the genomic binding sites for Pou5f3 at 512 cell stage and 50% epiboly and Sox2 at 50% epiboly stage using ChIP-seq. For Pou5f3 Chip-seq, MZspg mutant embryos, injected with 25 pg/embryo synthetic Flag-Pou5f3 mRNA and anti-Flag antibody were used. For Sox2 ChIp-seq, AB/TL zebrafish embryos and anti-Sox2 antibody were used. 76-basepair single-end reads of 50% epiboly Pou5f3 (twice, to reach the deeper read density) and Sox2 ChIP-seq and input control were sequenced the Genome Analyzer IIx (Illumina), resulting in 4 FASTQ files for ChIP-seq: one for Sox2 and for Input control, and two for Pou5f3. For the pre-MBT Pou5f3 ChIP samples, 51-basepair single-end reads were sequenced by GATC Biotech AG using a HighSeq 2000 (Illumina), resulting in 1 additional FASTQ file for ChIP-seq

Is public: True True False

Added by User: Onichtchouk

Date Added: May 30, 2016, 5:20 a.m.

Series Last Edited: Nov. 7, 2018, 5:15 a.m.