RNA-seq data of posterior body: Regulation of posterior body and ectodermal morphogenesis in zebrafish by localized Yap1 and Wwtr1
Case Control
10.7554/eLife.31065
GSE102606
The vertebrate embryo undergoes a series of dramatic morphological changes as the body extends to form the complete anterior-posterior axis during the somite-forming stages. We show that the Hippo pathway transcriptional coactivators Yap1 and Wwtr1 are specifically localized to the ectoderm and notochord, and play a critical and unexpected role in posterior body extension by regulating the assembly of Fibronectin underneath the ectoderm and surrounding the notochord. We also find that Yap1/Wwtr1, also acting through Fibronectin, have an essential role in the ectodermal morphogenesis necessary to form the initial dorsal and ventral fins, a process that had been thought to involve bending of an epithelial sheet, but which we now show involves active cell migration. Our results reveal how the Hippo pathway transcriptional program, localized to two specific tissues, acts to control essential morphological events in the vertebrate embryo.
True
mickael.dong
March 6, 2018, 5:42 a.m.
March 12, 2018, 3:54 a.m.
| Biosample ID | Series | biosample_type | biosample_lab | genetic_background | stage | Controlled By | anatomical_term | Hours Post Fertilization | Mutation Description |
|---|---|---|---|---|---|---|---|---|---|
| DCD007101BS | DCD000399SR | tissue | Stainier Lab, Max Planck Institute For Heart and Lung Research | crossbreed | 14-19 somites | tail bud | 16 | — |
| Applied Assay ID | Biosample Replicate | Assay |
|---|---|---|
| DCD002175AA | DCD013766BR | RNA-seq_Stainier_Lab_0001AS |
| Sequencing ID | Technical Replicate | Sequencing Lab | Platform | Instrument | Chemistry Version | Paired End | Strand | Sequencing Date |
|---|---|---|---|---|---|---|---|---|
| DCD002551SQ | DCD002577TR | Stainier Lab, Max Planck Institute For Heart and Lung Research | Illumina | NextSeq 500 High-Output | Truseq Stranded mRNA library preparation kit | ✔ | unstranded | 08/14/2017 |
| Applied Assay ID | Biosample Replicate | Assay |
|---|---|---|
| DCD002176AA | DCD013767BR | RNA-seq_Stainier_Lab_0001AS |
| Sequencing ID | Technical Replicate | Sequencing Lab | Platform | Instrument | Chemistry Version | Paired End | Strand | Sequencing Date |
|---|---|---|---|---|---|---|---|---|
| DCD002552SQ | DCD002578TR | Stainier Lab, Max Planck Institute For Heart and Lung Research | Illumina | NextSeq 500 High-Output | Truseq Stranded mRNA library preparation kit | ✔ | unstranded | 08/14/2017 |
| Biosample ID | Series | biosample_type | biosample_lab | genetic_background | stage | Controlled By | anatomical_term | Treatment | Hours Post Fertilization | Mutation Description |
|---|---|---|---|---|---|---|---|---|---|---|
| DCD007102BS | DCD000399SR | tissue | Stainier Lab, Max Planck Institute For Heart and Lung Research | crossbreed | 14-19 somites | DCD007101BS | tail bud | — | 16 | yap1 -/- ; wwtr1 -/- |
| Applied Assay ID | Biosample Replicate | Assay |
|---|---|---|
| DCD002177AA | DCD013768BR | RNA-seq_Stainier_Lab_0001AS |
| Sequencing ID | Technical Replicate | Sequencing Lab | Platform | Instrument | Chemistry Version | Paired End | Strand | Sequencing Date |
|---|---|---|---|---|---|---|---|---|
| DCD002553SQ | DCD002579TR | Stainier Lab, Max Planck Institute For Heart and Lung Research | Illumina | NextSeq 500 High-Output | Truseq Stranded mRNA library preparation kit | ✔ | unstranded | 08/14/2017 |
| Applied Assay ID | Biosample Replicate | Assay | Controlled By |
|---|---|---|---|
| DCD002178AA | DCD013769BR | RNA-seq_Stainier_Lab_0001AS |
| Sequencing ID | Technical Replicate | Sequencing Lab | Platform | Instrument | Chemistry Version | Paired End | Strand | Maximum Read Length | Sequencing Date |
|---|---|---|---|---|---|---|---|---|---|
| DCD002554SQ | DCD002580TR | Stainier Lab, Max Planck Institute For Heart and Lung Research | Illumina | NextSeq 500 High-Output | Truseq Stranded mRNA library preparation kit | ✔ | unstranded | — | 08/14/2017 |