Series Summary for DCD000399SR

RNA-seq data of posterior body: Regulation of posterior body and ectodermal morphogenesis in zebrafish by localized Yap1 and Wwtr1

Case Control

10.7554/eLife.31065

GSE102606

The vertebrate embryo undergoes a series of dramatic morphological changes as the body extends to form the complete anterior-posterior axis during the somite-forming stages. We show that the Hippo pathway transcriptional coactivators Yap1 and Wwtr1 are specifically localized to the ectoderm and notochord, and play a critical and unexpected role in posterior body extension by regulating the assembly of Fibronectin underneath the ectoderm and surrounding the notochord. We also find that Yap1/Wwtr1, also acting through Fibronectin, have an essential role in the ectodermal morphogenesis necessary to form the initial dorsal and ventral fins, a process that had been thought to involve bending of an epithelial sheet, but which we now show involves active cell migration. Our results reveal how the Hippo pathway transcriptional program, localized to two specific tissues, acts to control essential morphological events in the vertebrate embryo.

True

mickael.dong

March 6, 2018, 5:42 a.m.

March 12, 2018, 3:54 a.m.

Biosample ID Series biosample_type biosample_lab genetic_background
DCD007101BS DCD000399SR tissue Stainier Lab, Max Planck Institute For Heart and Lung Research crossbreed
Applied Assay ID Biosample Replicate Assay
DCD002175AA DCD013766BR RNA-seq_Stainier_Lab_0001AS
Sequencing ID Technical Replicate Sequencing Lab Platform Instrument Chemistry Version Paired End Strand Sequencing Date
DCD002551SQ DCD002577TR Stainier Lab, Max Planck Institute For Heart and Lung Research Illumina NextSeq 500 High-Output Truseq Stranded mRNA library preparation kit unstranded 08/14/2017
DCD008103DT: FASTQ
Applied Assay ID Biosample Replicate Assay
DCD002176AA DCD013767BR RNA-seq_Stainier_Lab_0001AS
Sequencing ID Technical Replicate Sequencing Lab Platform Instrument Chemistry Version Paired End Strand Sequencing Date
DCD002552SQ DCD002578TR Stainier Lab, Max Planck Institute For Heart and Lung Research Illumina NextSeq 500 High-Output Truseq Stranded mRNA library preparation kit unstranded 08/14/2017
DCD008104DT: FASTQ
Biosample ID Series biosample_type biosample_lab genetic_background
DCD007102BS DCD000399SR tissue Stainier Lab, Max Planck Institute For Heart and Lung Research crossbreed
Applied Assay ID Biosample Replicate Assay
DCD002177AA DCD013768BR RNA-seq_Stainier_Lab_0001AS
Sequencing ID Technical Replicate Sequencing Lab Platform Instrument Chemistry Version Paired End Strand Sequencing Date
DCD002553SQ DCD002579TR Stainier Lab, Max Planck Institute For Heart and Lung Research Illumina NextSeq 500 High-Output Truseq Stranded mRNA library preparation kit unstranded 08/14/2017
DCD008105DT: FASTQ
Applied Assay ID Biosample Replicate Assay
DCD002178AA DCD013769BR RNA-seq_Stainier_Lab_0001AS
Sequencing ID Technical Replicate Sequencing Lab Platform Instrument Chemistry Version Paired End Strand Sequencing Date
DCD002554SQ DCD002580TR Stainier Lab, Max Planck Institute For Heart and Lung Research Illumina NextSeq 500 High-Output Truseq Stranded mRNA library preparation kit unstranded 08/14/2017
DCD008106DT: FASTQ